embr.201642825.pdf (1.93 MB)
Structural studies of RFCCtf18 reveal a novel chromatin recruitment role for Dcc1
journal contributionposted on 2020-10-15, 16:38 authored by Benjamin O Wade, Hon Wing Liu, Catarina P Samora, Frank Uhlmann, Martin R Singleton
Replication factor C complexes load and unload processivity clamps from DNA and are involved in multiple DNA replication and repair pathways. The RFCCtf18 variant complex is required for activation of the intra-S-phase checkpoint at stalled replication forks and aids the establishment of sister chromatid cohesion. Unlike other RFC complexes, RFCCtf18 contains two non-Rfc subunits, Dcc1 and Ctf8. Here, we present the crystal structure of the Dcc1-Ctf8 heterodimer bound to the C-terminus of Ctf18. We find that the C-terminus of Dcc1 contains three-winged helix domains, which bind to both ssDNA and dsDNA We further show that these domains are required for full recruitment of the complex to chromatin, and correct activation of the replication checkpoint. These findings provide the first structural data on a eukaryotic seven-subunit clamp loader and define a new biochemical activity for Dcc1.
Ctf18Dcc1S‐phase checkpointX‐ray crystallographysister chromatid cohesionAmino Acid SequenceChromatinDNA-Binding ProteinsModels, MolecularProtein BindingProtein ConformationProtein Interaction Domains and MotifsProtein MultimerizationSaccharomyces cerevisiae ProteinsStructure-Activity RelationshipUhlmann FC001198Singleton FC001155SB-ackPRT-ackCB-ackDevelopmental Biology0601 Biochemistry and Cell Biology