Single Molecule Localisation Microscopyand Image Analysis to investigatethe sarcomere at the nanoscale

<p dir="ltr">The sarcomere</p><p dir="ltr">The sarcomere is the base contractile unit of muscle. With a length of ~2μm, interesting sub-sarcomeric structure is at the nanoscale and super-resolution (SR) microscopy is needed to image it and understand it. Electron Microscopy is limited in the denser regions made up of very similar domains; hence SR fluorescence microscopy provides nanoscale resolution imaging with high molecular specificity labelling. SMLM is at the forefront of SR-fluorescence microscopy, and is therefore an important tool for resolving substructure at the nanoscale.</p><p dir="ltr"><br></p><p dir="ltr">Poster presented as part of the Crick BioImage Analysis Symposium 2025.</p><p dir="ltr">Permission has been given by authors to upload to Crick Figshare. Copyright remains with the original authors.</p>