SPARCS, a platform for genome-scale CRISPR screening for subcellular spatial phenotypes

Poster presented as part of the Crick BioImage Analysis Symposium 2023.

Forward genetic screening associates phenotypes with genotypes by randomly inducing mutations and then identifying those that result in phenotypic changes of interest. SPARCS is a platform for genetic screens on subcellular spatial phenotypes acquired by microscopy. SPARCS uses automated high-speed laser microdissection to physically isolate phenotypic variants in situ from virtually unlimited library sizes. To demonstrate the potential of SPARCS we performed a genome-wide CRISPR-KO screen on autophagosome formation in 40 million cells.

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