SPARCS, a platform for genome-scale CRISPR screening for subcellular spatial phenotypes

<p dir="ltr">Poster presented as part of the Crick BioImage Analysis Symposium 2023.</p><p dir="ltr">Forward genetic screening associates phenotypes with genotypes by randomly inducing mutations and then identifying those that result in phenotypic changes of interest. SPARCS is a platform for genetic screens on subcellular spatial phenotypes acquired by microscopy. SPARCS uses automated high-speed laser microdissection to physically isolate phenotypic variants in situ from virtually unlimited library sizes. To demonstrate the potential of SPARCS we performed a genome-wide CRISPR-KO screen on autophagosome formation in 40 million cells.</p><p dir="ltr"><br></p><p dir="ltr"><i>Permission has been given by authors to upload to Crick Figshare. Copyright remains with the original authors.</i></p>