Quantitative Assessment of Clearing Protocol Efficacy for Multi-Dimensional Microscopy

<p dir="ltr">Light scattering properties of biological tissue often limit imaging depth and resolution when acquiring images; optical tissue clearing significantly reduces light scattering, boosting the signal-to-noise ratio and providing crucial spatial information for deep imaging. Here, we present a user-friendly, open-source analytical pipeline for assessing tissue clearing efficacy, and illustrate our pipeline with a simple proof of concept. This pipeline provides a standard framework for the quantitative analysis of clearing protocols, allowing life science researchers to select an optimal protocol for their specific needs.</p><p dir="ltr">Poster presented as part of the Crick BioImage Analysis Symposium 2025.</p><p dir="ltr">Permission has been given by authors to upload to Crick Figshare. Copyright remains with the original authors.</p>