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Is MINFLUX the right method to explore biology at the nanoscale?

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posted on 2023-03-03, 14:22 authored by Kirti PrakashKirti Prakash

Poster presented as part of the Crick BioImage Analysis Symposium

MINFLUX is a promising new development in single-molecule localization microscopy, claiming a resolution of 1-3 nm in living and fixed biological specimens. While MINFLUX can achieve very high localisation precision, quantitative analysis of reported results leads us to dispute the resolution claim and question reliability for imaging sub-100-nm structural features, in its current state. 


1. Prakash and Curd. Assessment of 3D MINFLUX data for quantitative structural biology in cells. bioRxiv (2021) (Primary reference) 

2. Gwosch et al. Assessment of 3D MINFLUX data for quantitative structural biology in cells revisited. bioRxiv (2022) 

3. Prakash. At the molecular resolution with MINFLUX?. Philosophical Transactions of the Royal Society A (2022) 

4. Balzarotti et al. Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes. Science (2017) 

5. Gwosch et al. MINFLUX nanoscopy delivers 3d multicolor nanometer resolution in cells. Nature methods (2020) 

6. Löschberger et al. Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore. JCS (2012) 

7. Thevathasan et al. Nuclear pores as versatile reference standards for quantitative superresolution microscopy. Nature methods (2019) 

8. Curd et al. Nanoscale pattern extraction from relative positions of sparse 3d localizations. Nano letters (2020) 

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