Enhanced Bacterial Cytological Profiling with super resolution techniques and fluorescent D-amino acids

• Bacterial cytological profiling (BCP) is a quick one step assay to help determine antibiotic mode of action (MoA)
• Bacteria are treated with an antibiotic and stained with fluorescent dyes associated with different cellular targets (e.g. DNA, membrane)
• Features extracted by image analysis are processed using machine learning to associate cytological profiles with specific MoAs of known antibioitcs
• Novel antibiotics can then be analysed and associated with known or novel pathways
• Dyes used are FM 4-64FX, a fixable membrane stain, DAPI, a membrane permeable DNA stain, and Sytox Green, a membrane integrity indicator

Poster presented as part of the Crick BioImage Analysis Symposium 2024.

Permission has been given by authors to upload to Crick Figshare. Copyright remains with the original authors.