Combining assessment methods to gain new insights into SMLM reconstruction accuracy

<p dir="ltr">Single Molecule Localisation Microscopy (SMLM) has become a common method in Bio-imaging. Despite most users being increasingly aware that factors other than the localisation precision (such as bias/artefacts) contribute to resolution, a universal way of assessing reconstruction quality does not yet exist.Currently the three most effective tools for assessing SMLM images are HAWKMAN, SQUIRREL and FRC, each assessing different aspects of the reconstruction.However, the results of these tools can be interdependent, complicating the assessment of reconstruction quality. Here we demonstrate a method and a software tool that in corporates slightly modified versions of these techniques into a combined assessment of reconstruction quality and resolution.</p><p dir="ltr"><br></p><p dir="ltr">Poster presented as part of the Crick BioImage Analysis Symposium 2025.</p><p dir="ltr">Permission has been given by authors to upload to Crick Figshare. Copyright remains with the original authors.</p>