Analysis of Liver Sinusoidal Endothelial Cell fenestration from 3D electron microscopy data

Pohar, Cécile; Rekik, Yousr; Gallet, Benoit; Tillet, Emmanuelle; Castan, Agnès; Chevallet, Mireille; Vigano, Nicola; Jouneau, Pierre-Henri; Deniaud, Aurélien

doi:10.25418/crick.24624864.v1

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Analysis of Liver Sinusoidal Endothelial Cell fenestration from 3D electron microscopy data

poster

posted on 2023-11-29, 13:02 authored by Cécile Pohar, Yousr Rekik, Benoit Gallet, Emmanuelle Tillet, Agnès Castan, Mireille Chevallet, Nicola Vigano, Pierre-Henri Jouneau, Aurélien Deniaud

Poster presented as part of the Crick BioImage Analysis Symposium 2023.

3D electron microscopy (3D-M) enables to obtain the ultrastructure of biological samples. Liver Sinusoidal Endothelial Cell fenestration (LSEC) forms an interface that allows the transport of essential molecules between blood and hepatocytes. LSEC fenestration can be characterised by Focused Ion Beam - Scanning Electron Microscope (FIB-SEM) at nanometer resolution. Analysing how Bone Morphogenetic Protein 9 (BMP9) affects the structure of LSEC is essential to understand the role of BMP9.

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