mTOR independent alteration in ULK1 Ser758 phosphorylation following chronic LRRK2 kinase inhibition
journal contributionposted on 27.08.2020, 15:41 authored by Claudia Manzoni, Adamantios Mamais, Sybille Dihanich, Marc PM Soutar, Helene Plun-Favreau, Rina Bandopadhyay, Rosella Abeti, Paola Giunti, John Hardy, Mark Cookson, Sharon A Tooze, Patrick A Lewis
Unc-51 Like Kinase 1 (ULK1) is a critical regulator of the biogenesis of autophagosomes, the central component of the catabolic macroautophagy pathway. Regulation of ULK1 activity is dependent upon several phosphorylation events acting to repress or activate the enzymatic function of this protein. Phosphorylation of Ser758 ULK1 has been linked to repression of autophagosome biogenesis and was thought to be exclusively dependent upon mTOR complex 1 kinase activity. In the present study, a novel regulation of Ser758 ULK1 phosphorylation is reported following prolonged inhibition of the Parkinson's disease linked protein leucine rich repeat kinase 2 (LRRK2). Here, modulation of Ser758 ULK1 phosphorylation following LRRK2 inhibition is decoupled from the repression of autophagosome biogenesis and independent of mTOR complex 1 activity.
Leucin Rich Repeat Kinase 2 (LRRK2)Parkinson diseaseUnc-51 Like Kinase 1 (ULK1)autophagycancermammalian Target Of Rapamycin (mTOR)AnimalsAutophagosomesAutophagy-Related Protein-1 HomologCell LineLeucine-Rich Repeat Serine-Threonine Protein Kinase-2Mechanistic Target of Rapamycin Complex 1PhosphorylationRatsSerineTOR Serine-Threonine KinasesTooze FC001187Biochemistry & Molecular Biology0601 Biochemistry and Cell Biology