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Download fileThe blood transcriptome of experimental melioidosis reflects disease severity and shows considerable similarity with the human disease
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posted on 2020-07-15, 12:01 authored by Laura Conejero, Krzysztof Potempa, Christine M Graham, Natasha Spink, Simon Blankley, Francisco J Salguero, Rungnapa Pankla-Sranujit, Prasong Khaenam, Jacques F Banchereau, Virginia Pascual, Damien Chaussabel, Ganjana Lertmemongkolchai, Anne O'Garra, Gregory J BancroftMelioidosis, a severe human disease caused by the bacterium Burkholderia pseudomallei, has a wide spectrum of clinical manifestations ranging from acute septicemia to chronic localized illness or latent infection. Murine models have been widely used to study the pathogenesis of infection and to evaluate novel therapies or vaccines, but how faithfully they recapitulate the biology of human melioidosis at a molecular level is not known. In this study, mice were intranasally infected with either high or low doses of B. pseudomallei to generate either acute, chronic, or latent infection and host blood and tissue transcriptional profiles were generated. Acute infection was accompanied by a homogeneous signature associated with induction of multiple innate immune response pathways, such as IL-10, TREM1, and IFN signaling, largely found in both blood and tissue. The transcriptional profile in blood reflected the heterogeneity of chronic infection and quantitatively reflected the severity of disease. Genes associated with fibrosis and tissue remodeling, including matrix metalloproteases and collagen, were upregulated in chronically infected mice with severe disease. Transcriptional signatures of both acute and chronic melioidosis revealed upregulation of iNOS in tissue, consistent with the expression of IFN-γ, but also Arginase-1, a functional antagonist of the iNOS pathway, and was confirmed by immunohistochemistry. Comparison of these mouse blood datasets by pathway and modular analysis with the blood transcriptional signature of patients with melioidosis showed that many genes were similarly perturbed, including Arginase-1, IL-10, TREM1, and IFN signaling, revealing the common immune response occurring in both mice and humans.
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AnimalsArginaseBurkholderia pseudomalleiDisease Models, AnimalFemaleGene Expression ProfilingHumansImmunity, InnateInterferon-gammaInterleukin-10LungMatrix Metalloproteinase 9MelioidosisMembrane GlycoproteinsMiceMice, Inbred C57BLNitric Oxide Synthase Type IIReceptors, ImmunologicSignal TransductionTranscriptomeTriggering Receptor Expressed on Myeloid Cells-1O'Garra U117565642HTS-ackImmunology1107 Immunology