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The aryl hydrocarbon receptor contributes to tissue adaptation of intestinal eosinophils in mice

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posted on 2022-03-07, 12:59 authored by Nicola Laura Diny, Barbora Schonfeldova, Michael Shapiro, Matthew L Winder, Sunita Varsani-Brown, Brigitta Stockinger
Eosinophils are potent sources of inflammatory and toxic mediators, yet they reside in large numbers in the healthy intestine without causing tissue damage. We show here that intestinal eosinophils were specifically adapted to their environment and underwent substantial transcriptomic changes. Intestinal eosinophils upregulated genes relating to the immune response, cell–cell communication, extracellular matrix remodeling, and the aryl hydrocarbon receptor (AHR), a ligand-activated transcription factor with broad functions in intestinal homeostasis. Eosinophils from AHR-deficient mice failed to fully express the intestinal gene expression program, including extracellular matrix organization and cell junction pathways. AHR-deficient eosinophils were functionally impaired in the adhesion to and degradation of extracellular matrix, were more prone to degranulation, and had an extended life span. Lack of AHR in eosinophils had wider effects on the intestinal immune system, affecting the T cell compartment in nave and helminth-infected mice. Our study demonstrates that the response to environmental triggers via AHR partially shapes tissue adaptation of eosinophils in the small intestine.


Crick (Grant ID: 10019, Grant title: STP BRF - Genetic Modification Service) Crick (Grant ID: 10159, Grant title: Stockinger FC001159) Wellcome Trust (Grant ID: 210556/Z/18/Z, Grant title: WT 210556/Z/18/Z)


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