Suppression of NYVAC infection in HeLa cells requires RNase L but is independent of Protein Kinase R activity
journal contributionposted on 2020-07-27, 11:17 authored by Mercedes Fernández-Escobar, José Luis Nájera, Sara Baldanta, Dolores Rodriguez, Michael Way, Mariano Esteban, Susana Guerra
Protein kinase R (PKR) and RNase L are host cell components that function to contain viral spread after infections. In this study, we analyzed the role of both proteins in the abortive infection of human HeLa cells with the poxvirus strain NYVAC, for which an inhibition of viral A27L and B5R gene expression is described. Specifically, the translation of these viral genes is independent of PKR activation, but their expression is dependent on the RNase L activity.
EndoribonucleasesEpithelial CellsHeLa CellsHost-Pathogen InteractionsHumansMembrane GlycoproteinsProtein BiosynthesisVaccinia virusViral Envelope ProteinsViral ProteinseIF-2 KinaseHela CellsWay FC001209Virology06 Biological Sciences07 Agricultural and Veterinary Sciences11 Medical and Health Sciences