The Francis Crick Institute
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Suppression of NYVAC infection in HeLa cells requires RNase L but is independent of Protein Kinase R activity

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journal contribution
posted on 2020-07-27, 11:17 authored by Mercedes Fernández-Escobar, José Luis Nájera, Sara Baldanta, Dolores Rodriguez, Michael Way, Mariano Esteban, Susana Guerra
Protein kinase R (PKR) and RNase L are host cell components that function to contain viral spread after infections. In this study, we analyzed the role of both proteins in the abortive infection of human HeLa cells with the poxvirus strain NYVAC, for which an inhibition of viral A27L and B5R gene expression is described. Specifically, the translation of these viral genes is independent of PKR activation, but their expression is dependent on the RNase L activity.