The Francis Crick Institute
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Substantially thinner internal granular layer and reduced molecular layer surface in the cerebellar cortex of the Tc1 mouse model of Down Syndrome - a comprehensive morphometric analysis with active staining contrast-enhanced MRI.

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journal contribution
posted on 2020-09-08, 14:04 authored by Da Ma, Manuel J Cardoso, Maria A Zuluaga, Marc Modat, Nick M Powell, Frances K Wiseman, Jon O Cleary, Benjamin Sinclair, Ian F Harrison, Bernard Siow, Karteek Popuri, Sieun Lee, Joanne A Matsubara, Marinko V Sarunic, Mirza Faisal Beg, Victor LJ Tybulewicz, Elizabeth MC Fisher, Mark F Lythgoe, S Ebastien Ourselin
Down Syndrome is a chromosomal disorder that affects the development of cerebellar cortical lobules. Impaired neurogenesis in the cerebellum varies among different types of neuronal cells and neuronal layers. In this study, we developed an imaging analysis framework that utilizes gadolinium-enhanced ex vivo mouse brain MRI. We extracted the middle Purkinje layer of the mouse cerebellar cortex, enabling the estimation of the volume, thickness, and surface area of the entire cerebellar cortex, the internal granular layer, and the molecular layer in the Tc1 mouse model of Down Syndrome. The morphometric analysis of our method revealed that a larger proportion of the cerebellar thinning in this model of Down Syndrome resided in the inner granule cell layer, while a larger proportion of the surface area shrinkage was in the molecular layer.


Crick (Grant ID: 10194, Grant title: Tybulewicz FC001194) Wellcome Trust (Grant ID: 098328/B/12/Z, Grant title: WT 098328/B/12/Z)