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Structural characterisation of the Chaetomium thermophilum Chl1 helicase.

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journal contribution
posted on 2021-05-17, 13:44 authored by Zuzana Hodáková, Andrea Nans, Simone Kunzelmann, Shahid Mehmood, Ian Taylor, Frank Uhlmann, Peter Cherepanov, Martin R Singleton
Chl1 is a member of the XPD family of 5'-3' DNA helicases, which perform a variety of roles in genome maintenance and transmission. They possess a variety of unique structural features, including the presence of a highly variable, partially-ordered insertion in the helicase domain 1. Chl1 has been shown to be required for chromosome segregation in yeast due to its role in the formation of persistent chromosome cohesion during S-phase. Here we present structural and biochemical data to show that Chl1 has the same overall domain organisation as other members of the XPD family, but with some conformational alterations. We also present data suggesting the insert domain in Chl1 regulates its DNA binding.

Funding

Crick (Grant ID: 10061, Grant title: Cherepanov FC001061) Crick (Grant ID: 10155, Grant title: Singleton FC001155) Crick (Grant ID: 10011, Grant title: STP Proteomics) Crick (Grant ID: 10015, Grant title: STP Structural Biology) Crick (Grant ID: 10178, Grant title: Taylor,I FC001178) Crick (Grant ID: 10198, Grant title: Uhlmann FC001198)

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