The Francis Crick Institute
jbio.202100144 (1).pdf (745.45 kB)

Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast.

Download (745.45 kB)
journal contribution
posted on 2021-12-08, 12:46 authored by Ranjan Kalita, William Flanagan, Jonathan Lightley, Sunil Kumar, Yuriy Alexandrov, Edwin Garcia, Mark Hintze, Michalis Barkoulas, Chris Dunsby, Paul MW French
We present a robust, low-cost single-shot implementation of differential phase microscopy utilising a polarisation-sensitive camera to simultaneously acquire four images from which phase contrast images can be calculated. This polarisation-resolved differential phase contrast (pDPC) microscopy technique can be easily integrated with fluorescence microscopy.