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SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay

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journal contribution
posted on 15.06.2021, 13:10 by MD Buck, EZ Poirier, C Reis e Sousa, A Cardoso, B Frederico, J Canton, S Barrell, R Beale, R Byrne, S Caidan, M Crawford, L Cubitt, S Gandhi, R Goldstone, PR Grant, K Gulati, S Hindmarsh, M Howell, M Hubank, R Instrell, M Jiang, G Kassiotis, WT Lu, JI MacRae, I Martini, D Miller, D Moore, E Nastouli, J Nicod, L Nightingale, J Olsen, A Oomatia, N O'Reilly, A Rideg, OR Song, A Strange, C Swanton, S Turajlic, M Wu, Caetano Reis e Sousa, The Crick COVID-19 Consortium
The ongoing pandemic of SARS-CoV-2 calls for rapid and cost-effective methods to accurately identify infected individuals. The vast majority of patient samples is assessed for viral RNA presence by RT-qPCR. Our biomedical research institute, in collaboration between partner hospitals and an accredited clinical diagnostic laboratory, established a diagnostic testing pipeline that has reported on more than 252,000 RT-qPCR results since its commencement at the beginning of April 2020. However, due to ongoing demand and competition for critical resources, alternative testing strategies were sought. In this work, we present a clinically-validated procedure for high-throughput SARS-CoV-2 detection by RT-LAMP that is robust, reliable, repeatable, specific, and inexpensive.

Funding

Crick (Grant ID: 10827, Grant title: Beale FC001827) Crick (Grant ID: 10099, Grant title: Kassiotis FC001099) Crick (Grant ID: 10136, Grant title: Reis e Sousa FC001136) Crick (Grant ID: 10001, Grant title: STP Advanced Sequencing) Crick (Grant ID: 10019, Grant title: STP BRF - Genetic Modification Service) Crick (Grant ID: 10008, Grant title: STP High Throughput Screening) Crick (Grant ID: 10012, Grant title: STP Metabolomics) Crick (Grant ID: 10013, Grant title: STP Peptide Chemistry) Crick (Grant ID: 10233, Grant title: STP Scientific Computing) Crick (Grant ID: 10169, Grant title: Swanton FC001169) Crick (Grant ID: 10988, Grant title: Turajlic FC001988)

History