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Download fileRepression of divergent noncoding transcription by a sequence-specific transcription factor
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posted on 2020-10-01, 14:49 authored by Andrew CK Wu, Harshil Patel, Minghao Chia, Fabien Moretto, David Frith, Ambrosius P Snijders, Folkert J van WervenMany active eukaryotic gene promoters exhibit divergent noncoding transcription, but the mechanisms restricting expression of these transcripts are not well understood. Here, we demonstrate how a sequence-specific transcription factor represses divergent noncoding transcription at highly expressed genes in yeast. We find that depletion of the transcription factor Rap1 induces noncoding transcription in a large fraction of Rap1-regulated gene promoters. Specifically, Rap1 prevents transcription initiation at cryptic promoters near its binding sites, which is uncoupled from transcription regulation in the protein-coding direction. We further provide evidence that Rap1 acts independently of previously described chromatin-based mechanisms to repress cryptic or divergent transcription. Finally, we show that divergent transcription in the absence of Rap1 is elicited by the RSC chromatin remodeler. We propose that a sequence-specific transcription factor limits access of basal transcription machinery to regulatory elements and adjacent sequences that act as divergent cryptic promoters, thereby providing directionality toward productive transcription.
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RSCRap1directionalitydivergentnoncoding RNApromoterrepressiontranscriptiontranscription factoryeastBinding SitesChromatin Assembly and DisassemblyGene Expression Regulation, FungalPromoter Regions, GeneticProtein BindingProtein Interaction Domains and MotifsRNA, FungalRNA, UntranslatedRibosomal ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTelomere-Binding ProteinsTranscription FactorsTranscription, Geneticvan Werven FC001203CBPRTAS-ackGEP-ackDevelopmental Biology06 Biological Sciences11 Medical and Health Sciences