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Reactive oxygen species localization programs inflammation to clear microbes of different size
journal contributionposted on 2020-10-15, 16:39 authored by Annika Warnatsch, Theodora-Dorita Tsourouktsoglou, Nora Branzk, Qian Wang, Susanna Reincke, Susanne Herbst, Maximiliano Gutierrez, Venizelos Papayannopoulos
How the number of immune cells recruited to sites of infection is determined and adjusted to differences in the cellular stoichiometry between host and pathogen is unknown. Here, we have uncovered a role for reactive oxygen species (ROS) as sensors of microbe size. By sensing the differential localization of ROS generated in response to microbes of different size, neutrophils tuned their interleukin (IL)-1β expression via the selective oxidation of NF-κB, in order to implement distinct inflammatory programs. Small microbes triggered ROS intracellularly, suppressing IL-1β expression to limit neutrophil recruitment as each phagocyte eliminated numerous pathogens. In contrast, large microbes triggered ROS extracellularly, amplifying IL-1β expression to recruit numerous neutrophils forming cooperative clusters. Defects in ROS-mediated microbe size sensing resulted in large neutrophil infiltrates and clusters in response to small microbes that contribute to inflammatory disease. These findings highlight the impact of ROS localization on signal transduction.
Candida albicanscellular stoichiometryclusterfungal infectioninflammationinterleukin-1βmicrobe sizeneutrophilreactive oxygen speciesswarmAnimalsBacteriaBacterial InfectionsDisease Models, AnimalFemaleHumansInflammationMaleMiceMice, Inbred C57BLMitosporic FungiMycosesNeutrophil InfiltrationNeutrophilsReactive Oxygen SpeciesPapayannopoulos FC001129Gutierrez FC001092GSKImmunology1107 Immunology