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Rare SH2B3 coding variants in lupus patients impair B cell tolerance and predispose to autoimmunity.

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journal contribution
posted on 2024-05-13, 10:28 authored by Yaoyuan Zhang, Rhiannon Morris, Grant J Brown, Ayla May D Lorenzo, Xiangpeng Meng, Nadia J Kershaw, Pamudika Kiridena, Gaétan Burgio, Simon Gross, Jean Y Cappello, Qian Shen, Hao Wang, Cynthia Turnbull, Tom Lea-Henry, Maurice Stanley, Zhijia Yu, Fiona D Ballard, Aaron Chuah, James C Lee, Ann-Maree Hatch, Anselm Enders, Seth L Masters, Alexander P Headley, Peter Trnka, Dominic Mallon, Jeffery T Fletcher, Giles D Walters, Mario Šestan, Marija Jelušić, Matthew C Cook, Vicki Athanasopoulos, David A Fulcher, Jeffrey J Babon, Carola G Vinuesa, Julia I Ellyard
Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease with a clear genetic component. While most SLE patients carry rare gene variants in lupus risk genes, little is known about their contribution to disease pathogenesis. Amongst them, SH2B3-a negative regulator of cytokine and growth factor receptor signaling-harbors rare coding variants in over 5% of SLE patients. Here, we show that unlike the variant found exclusively in healthy controls, SH2B3 rare variants found in lupus patients are predominantly hypomorphic alleles, failing to suppress IFNGR signaling via JAK2-STAT1. The generation of two mouse lines carrying patients' variants revealed that SH2B3 is important in limiting the number of immature and transitional B cells. Furthermore, hypomorphic SH2B3 was shown to impair the negative selection of immature/transitional self-reactive B cells and accelerate autoimmunity in sensitized mice, at least in part due to increased IL-4R signaling and BAFF-R expression. This work identifies a previously unappreciated role for SH2B3 in human B cell tolerance and lupus risk.


Crick (Grant ID: CC2219, Grant title: Lee CC2219) Crick (Grant ID: CC2228, Grant title: Vinuesa CC2228)