Plasma membrane association but not midzone recruitment of RhoGEF ECT2 is essential for cytokinesis
journal contributionposted on 2020-08-12, 13:44 authored by Kristýna Kotýnková, Kuan-Chung Su, Stephen C West, Mark Petronczki
Cytokinesis, the final step of cell division, begins with the formation of a cleavage furrow. How the mitotic spindle specifies the furrow at the equator in animal cells remains unknown. Current models propose that the concentration of the RhoGEF ECT2 at the spindle midzone and the equatorial plasma membrane directs furrow formation. Using chemical genetic and optogenetic tools, we demonstrate that the association of ECT2 with the plasma membrane during anaphase is required and sufficient for cytokinesis. Local membrane targeting of ECT2 leads to unilateral furrowing, highlighting the importance of local ECT2 activity. ECT2 mutations that prevent centralspindlin binding compromise concentration of ECT2 at the midzone and equatorial membrane but sustain cytokinesis. While the association of ECT2 with the plasma membrane is essential for cytokinesis, our data suggest that ECT2 recruitment to the spindle midzone is insufficient to account for equatorial furrowing and may act redundantly with yet-uncharacterized signals.
cell divisionchemical geneticscleavage planecontractile ringcytokinesiscytoskeletonmitosisoptogeneticsplasma membranespindle midzoneAnaphaseCell MembraneCytokinesisHeLa CellsHumansMicrotubule-Associated ProteinsMutationProtein BindingProto-Oncogene ProteinsSpindle ApparatusHela CellsWest FC0012120601 Biochemistry and Cell Biology