Observation of DNA intertwining along authentic budding yeast chromosomes
journal contributionposted on 2020-10-28, 14:04 authored by Ainhoa Mariezcurrena, Frank Uhlmann
DNA replication of circular genomes generates physically interlinked or catenated sister DNAs. These are resolved through transient DNA fracture by type II topoisomerases to permit chromosome segregation during cell division. Topoisomerase II is similarly required for linear chromosome segregation, suggesting that linear chromosomes also remain intertwined following DNA replication. Indeed, chromosome resolution defects are a frequent cause of chromosome segregation failure and consequent aneuploidies. When and where intertwines arise and persist along linear chromosomes are not known, owing to the difficulty of demonstrating intertwining of linear DNAs. Here, we used excision of chromosomal regions as circular "loop outs" to convert sister chromatid intertwines into catenated circles. This revealed intertwining at replication termination and cohesin-binding sites, where intertwines are thought to arise and persist but not to a greater extent than elsewhere in the genome. Intertwining appears to spread evenly along chromosomes but is excluded from heterochromatin. We found that intertwines arise before replication termination, suggesting that replication forks rotate during replication elongation to dissipate torsion ahead of the forks. Our approach provides previously inaccessible insight into the topology of eukaryotic chromosomes and illuminates a process critical for successful chromosome segregation.
DNA topologyS. cerevisiaechromosome segregationprecatenanessister chromatid intertwiningtopoisomerase IICell Cycle ProteinsChromosomal Proteins, Non-HistoneChromosome SegregationChromosomes, FungalDNA ReplicationDNA, FungalGenetic StructuresGenome, FungalHeterochromatinReplication OriginSaccharomyces cerevisiaeUhlmann FC001198Developmental Biology06 Biological Sciences11 Medical and Health Sciences17 Psychology and Cognitive Sciences