The Francis Crick Institute
1-s2.0-S2211124716304740-main.pdf (3.22 MB)

Multiomic analysis of the UV-induced DNA damage response

Download (3.22 MB)
journal contribution
posted on 2020-07-15, 08:54 authored by Stefan Boeing, Laura Williamson, Vesela Encheva, Ilaria Gori, Rebecca E Saunders, Rachael Instrell, Ozan Aygün, Marta Rodriguez-Martinez, Juston C Weems, Gavin P Kelly, Joan W Conaway, Ronald C Conaway, Aengus Stewart, Michael Howell, Ambrosius P Snijders, Jesper Q Svejstrup
In order to facilitate the identification of factors and pathways in the cellular response to UV-induced DNA damage, several descriptive proteomic screens and a functional genomics screen were performed in parallel. Numerous factors could be identified with high confidence when the screen results were superimposed and interpreted together, incorporating biological knowledge. A searchable database, bioLOGIC, which provides access to relevant information about a protein or process of interest, was established to host the results and facilitate data mining. Besides uncovering roles in the DNA damage response for numerous proteins and complexes, including Integrator, Cohesin, PHF3, ASC-1, SCAF4, SCAF8, and SCAF11, we uncovered a role for the poorly studied, melanoma-associated serine/threonine kinase 19 (STK19). Besides effectively uncovering relevant factors, the multiomic approach also provides a systems-wide overview of the diverse cellular processes connected to the transcription-related DNA damage response.