FEBS Letters - 2023 - Tooze - Membrane association of the ATG8 conjugation machinery emerges as a key regulatory feature.pdf (606.99 kB)
Membrane association of the ATG8 conjugation machinery emerges as a key regulatory feature for autophagosome biogenesis.
journal contributionposted on 2024-01-18, 11:05 authored by Sharon A Tooze, Wenxin Zhang, Gianmarco Lazzeri, Deepanshi Gahlot, Lipi Thukral, Roberto Covino, Taki Nishimura
Autophagy is a highly conserved intracellular pathway which is essential for survival in all eukaryotes. In healthy cells, autophagy is used to remove damaged intracellular components, which can be as simple as unfolded proteins or as complex as whole mitochondria. Once the damaged component is captured, the autophagosome engulfs it and closes, isolating the content from the cytoplasm. The autophagosome then fuses with the late endosome and/or lysosome to deliver its content to the lysosome for degradation. Formation of the autophagosome, sequestration or capture of content, and closure all require the ATG proteins, which constitute the essential core autophagy protein machinery. This brief "nutshell" will highlight recent data revealing the importance of small membrane associated domains in the ATG proteins. In particular, recent findings from two parallel studies reveal the unexpected key role of α-helical structures in the ATG8 conjugation machinery and ATG8s. These studies illustrate how unique membrane association modules can control the formation of autophagosomes.
Crick (Grant ID: CC2134, Grant title: Tooze CC2134)
Amphipathic α-helixATG3ATG8 lipidationautophagosome sizeAutophagycis-membrane associationMD simulationmembrane expansionamphipathic α-helixautophagyTooze CC21340304 Medicinal and Biomolecular Chemistry0601 Biochemistry and Cell Biology0603 Evolutionary BiologyBiochemistry & Molecular Biology3101 Biochemistry and cell biology