Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
journal contributionposted on 2020-07-17, 16:29 authored by Maike Frye, Andrea Taddei, Cathrin Dierkes, Ines Martinez-Corral, Matthew Fielden, Henrik Ortsäter, Jan Kazenwadel, Dinis P Calado, Pia Ostergaard, Marjo Salminen, Liqun He, Natasha L Harvey, Friedemann Kiefer, Taija Mäkinen
Tissue and vessel wall stiffening alters endothelial cell properties and contributes to vascular dysfunction. However, whether extracellular matrix (ECM) stiffness impacts vascular development is not known. Here we show that matrix stiffness controls lymphatic vascular morphogenesis. Atomic force microscopy measurements in mouse embryos reveal that venous lymphatic endothelial cell (LEC) progenitors experience a decrease in substrate stiffness upon migration out of the cardinal vein, which induces a GATA2-dependent transcriptional program required to form the first lymphatic vessels. Transcriptome analysis shows that LECs grown on a soft matrix exhibit increased GATA2 expression and a GATA2-dependent upregulation of genes involved in cell migration and lymphangiogenesis, including VEGFR3. Analyses of mouse models demonstrate a cell-autonomous function of GATA2 in regulating LEC responsiveness to VEGF-C and in controlling LEC migration and sprouting in vivo. Our study thus uncovers a mechanism by which ECM stiffness dictates the migratory behavior of LECs during early lymphatic development.
AnimalsCell MovementEndothelial CellsFemaleGATA2 Transcription FactorGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Knockdown TechniquesHumansLymphangiogenesisLymphatic VesselsMaleMiceMice, TransgenicPrimary Cell CultureRNA, Small InterferingVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-3Calado FC001057FC-ack