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Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2.
journal contributionposted on 2021-12-08, 14:14 authored by Hector Huerga Encabo, Rachel Ulferts, Aneesh Sharma, Rupert Beale, Dominique Bonnet
Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-PCR and colony forming unit assay.