Heteromeric RNP assembly at LINEs controls lineage-specific RNA processing
journal contributionposted on 2020-06-25, 15:21 authored by Jan Attig, Federico Agostini, Clare Gooding, Anob M Chakrabarti, Aarti Singh, Nejc Haberman, Julian A Zagalak, Warren Emmett, Christopher WJ Smith, Nicholas M Luscombe, Jernej Ule
Long mammalian introns make it challenging for the RNA processing machinery to identify exons accurately. We find that LINE-derived sequences (LINEs) contribute to this selection by recruiting dozens of RNA-binding proteins (RBPs) to introns. This includes MATR3, which promotes binding of PTBP1 to multivalent binding sites within LINEs. Both RBPs repress splicing and 3' end processing within and around LINEs. Notably, repressive RBPs preferentially bind to evolutionarily young LINEs, which are located far from exons. These RBPs insulate the LINEs and the surrounding intronic regions from RNA processing. Upon evolutionary divergence, changes in RNA motifs within LINEs lead to gradual loss of their insulation. Hence, older LINEs are located closer to exons, are a common source of tissue-specific exons, and increasingly bind to RBPs that enhance RNA processing. Thus, LINEs are hubs for the assembly of repressive RBPs and also contribute to the evolution of new, lineage-specific transcripts in mammals. VIDEO ABSTRACT.
CLIPLINE repeatsMATR3PTBP1alternative polyadenylationcryptic exonsevolutionexonogenesismultivalencysplicingAlternative SplicingAnimalsBinding SitesExonsHeLa CellsHeterogeneous-Nuclear RibonucleoproteinsHumansIntronsLong Interspersed Nucleotide ElementsMiceMutationNuclear Matrix-Associated ProteinsNucleotide MotifsPhylogenyPolyadenylationPolypyrimidine Tract-Binding ProteinProtein BindingProtein Interaction MappingRNARNA SplicingRNA-Binding ProteinsHela CellsLuscombe FC001110Ule - secDevelopmental Biology06 Biological Sciences11 Medical and Health Sciences