GABARAP activates ULK1 and traffics from the centrosome dependent on Golgi partners WAC and GOLGA2/GM130
journal contributionposted on 05.08.2020, 17:11 by Justin Joachim, Sharon A Tooze
WAC and GOLGA2/GM130 are 2 Golgi proteins that affect autophagy; however, their mechanism of action was unknown. We have shown that WAC binding to GOLGA2 at the Golgi displaces GABARAP from GOLGA2 to allow the maintenance of a nonlipidated centrosomal GABARAP pool. Centrosomal GABARAP can traffic to autophagic structures during starvation. In addition GABARAP specifically promotes ULK1 activation and this is independent of GABARAP lipidation but likely requires a LIR-mediated GABARAP-ULK1 interaction.
Atg8GABARAPGM130GOLGA2ULK1WACcentrosomegolgiphotoactivationAdaptor Proteins, Signal TransducingAnimalsAutoantigensAutophagyAutophagy-Related Protein-1 HomologCentrosomeGolgi ApparatusHumansMembrane ProteinsMicrotubule-Associated ProteinsTooze FC001187Biochemistry & Molecular Biology0601 Biochemistry and Cell Biology