GABARAP activates ULK1 and traffics from the centrosome dependent on Golgi partners WAC and GOLGA2 GM130.pdf (1.09 MB)
GABARAP activates ULK1 and traffics from the centrosome dependent on Golgi partners WAC and GOLGA2/GM130
journal contributionposted on 2020-08-05, 17:11 authored by Justin Joachim, Sharon A Tooze
WAC and GOLGA2/GM130 are 2 Golgi proteins that affect autophagy; however, their mechanism of action was unknown. We have shown that WAC binding to GOLGA2 at the Golgi displaces GABARAP from GOLGA2 to allow the maintenance of a nonlipidated centrosomal GABARAP pool. Centrosomal GABARAP can traffic to autophagic structures during starvation. In addition GABARAP specifically promotes ULK1 activation and this is independent of GABARAP lipidation but likely requires a LIR-mediated GABARAP-ULK1 interaction.
Atg8GABARAPGM130GOLGA2ULK1WACcentrosomegolgiphotoactivationAdaptor Proteins, Signal TransducingAnimalsAutoantigensAutophagyAutophagy-Related Protein-1 HomologCentrosomeGolgi ApparatusHumansMembrane ProteinsMicrotubule-Associated ProteinsTooze FC001187Biochemistry & Molecular Biology0601 Biochemistry and Cell Biology