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Fluorescence-activated nuclei negative sorting of neurons combined with single nuclei RNA sequencing to study the hippocampal neurogenic niche.
journal contributionposted on 2022-11-14, 10:39 authored by Thomas Kerloch, Tjaša Lepko, Kirill Shkura, François Guillemot, Sébastien Gillotin
Adult Hippocampal Neurogenesis (AHN), which consists of a lifelong maintenance of proliferative and quiescent neural stem cells (NSCs) within the sub-granular zone (SGZ) of the dentate gyrus (DG) and their differentiation from newly born neurons into granule cells in the granule cell layer, is well validated across numerous studies. Using genetically modified animals, particularly rodents, is a valuable tool to investigate signaling pathways regulating AHN and to study the role of each cell type that compose the hippocampal neurogenic niche. To address the latter, methods combining single nuclei isolation with next generation sequencing have had a significant impact in the field of AHN to identify gene signatures for each cell population. Further refinement of these techniques is however needed to phenotypically profile rarer cell populations within the DG. Here, we present a method that utilizes Fluorescence Activated Nuclei Sorting (FANS) to exclude most neuronal populations from a single nuclei suspension isolated from freshly dissected DG, by selecting unstained nuclei for the NeuN antigen, in order to perform single nuclei RNA sequencing (snRNA-seq). This method is a potential steppingstone to further investigate intercellular regulation of the AHN and to uncover novel cellular markers and mechanisms across species.
Crick (Grant ID: 10089, Grant title: Guillemot FC001089) Wellcome Trust (Grant ID: 106187/Z/14/Z, Grant title: WT 106187/Z/14/Z)