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Establishment of dsDNA-dsDNA interactions by the condensin complex.
journal contributionposted on 2023-11-09, 14:27 authored by Minzhe Tang, Georgii Pobegalov, Hideki Tanizawa, Zhuo A Chen, Juri Rappsilber, Maxim Molodtsov, Ken-ichi Noma, Frank Uhlmann
Condensin is a structural maintenance of chromosomes (SMC) complex family member thought to build mitotic chromosomes by DNA loop extrusion. However, condensin variants unable to extrude loops, yet proficient in chromosome formation, were recently described. Here, we explore how condensin might alternatively build chromosomes. Using bulk biochemical and single-molecule experiments with purified fission yeast condensin, we observe that individual condensins sequentially and topologically entrap two double-stranded DNAs (dsDNAs). Condensin loading transitions through a state requiring DNA bending, as proposed for the related cohesin complex. While cohesin then favors the capture of a second single-stranded DNA (ssDNA), second dsDNA capture emerges as a defining feature of condensin. We provide complementary in vivo evidence for DNA-DNA capture in the form of condensin-dependent chromatin contacts within, as well as between, chromosomes. Our results support a "diffusion capture" model in which condensin acts in mitotic chromosome formation by sequential dsDNA-dsDNA capture.
Crick (Grant ID: CC2125, Grant title: Molodtsov CC2125) Crick (Grant ID: CC2137, Grant title: Uhlmann CC2137) Wellcome Trust (Grant ID: 220244/Z/20/Z, Grant title: WT 220244/Z/20/Z)
biochemical reconstitutionChIA-PETchromosome formationcohesincondensinmitosisS. pombesingle-molecule microscopySMC complexesUhlmann CC2137Molodtsov CC2125CB-ackFERM-ackSchneider-Luftman, Deborah06 Biological Sciences11 Medical and Health SciencesDevelopmental Biology31 Biological sciences32 Biomedical and clinical sciences42 Health sciences