Enigma proteins regulate YAP mechanotransduction
journal contributionposted on 2020-07-15, 11:11 authored by Ahmed Elbediwy, Hannah Vanyai, Maria Diaz De La Loza, David Frith, Ambrosius P Snijders, Barry J Thompson
Human cells can sense mechanical stress acting upon integrin adhesions and respond by sending the YAP (also known as YAP1) and TAZ (also known as WWTR1) transcriptional co-activators to the nucleus to drive TEAD-dependent transcription of target genes. How integrin signaling activates YAP remains unclear. Here, we show that integrin-mediated mechanotransduction requires the Enigma and Enigma-like proteins (PDLIM7 and PDLIM5, respectively; denoted for the family of PDZ and LIM domain-containing proteins). YAP binds to PDLIM5 and PDLIM7 (hereafter PDLIM5/7) via its C-terminal PDZ-binding motif (PBM), which is essential for full nuclear localization and activity of YAP. Accordingly, silencing of PDLIM5/7 expression reduces YAP nuclear localization, tyrosine phosphorylation and transcriptional activity. The PDLIM5/7 proteins are recruited from the cytoplasm to integrin adhesions and F-actin stress fibers in response to force by binding directly to the key stress fiber component α-actinin. Thus, forces acting on integrins recruit Enigma family proteins to trigger YAP activation during mechanotransduction.This article has an associated First Person interview with the first author of the paper.
EnigmaHippoIntegrinMechanotransductionPDLIM5PDLIM7YAPAdaptor Proteins, Signal TransducingAnimalsCaco-2 CellsCytoskeletal ProteinsFibroblastsHEK293 CellsHumansIntegrinsLIM Domain ProteinsMechanotransduction, CellularMiceSignal TransductionTranscription FactorsThompson FC001180PRT06 Biological Sciences11 Medical and Health SciencesDevelopmental Biology