Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

Calder, Lesley J; Calcraft, Thomas; Hussain, Saira; Harvey, Ruth; Rosenthal, Peter B

doi:10.25418/crick.21542652.v1

s42003-022-04183-1.pdf (1.69 MB)

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Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

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posted on 2022-11-11, 12:56 authored by Lesley J Calder, Thomas Calcraft, Saira Hussain, Ruth Harvey, Peter B Rosenthal

SARS-CoV-2 is a lipid-enveloped Betacoronavirus and cause of the Covid-19 pandemic. To study the three-dimensional architecture of the virus, we perform electron cryotomography (cryo-ET) on SARS-Cov-2 virions and three variants revealing particles of regular cylindrical morphology. The ribonucleoprotein particles packaging the genome in the virion interior form a dense, double layer assembly with a cylindrical shape related to the overall particle morphology. This organisation suggests structural interactions important to virus assembly.

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Crick (Grant ID: 10143, Grant title: Rosenthal FC001143) Crick (Grant ID: 10030, Grant title: McCauley FC001030) Crick (Grant ID: 11104, Grant title: Bauer FC011104)

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https://doi.org/10.1038/s42003-022-04183-1

Electron cryotomography of SARS-CoV-2 virions reveals cylinder-shaped particles with a double layer RNP assembly

Funding

Crick (Grant ID: 10143, Grant title: Rosenthal FC001143) Crick (Grant ID: 10030, Grant title: McCauley FC001030) Crick (Grant ID: 11104, Grant title: Bauer FC011104)

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