The Francis Crick Institute
Kakui-Uhlmann2019_Protocol_EfficientDepletionOfFissionYea.pdf (318.7 kB)

Efficient depletion of fission yeast condensin by combined transcriptional repression and auxin-induced degradation.

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journal contribution
posted on 2020-06-25, 15:06 authored by Yasutaka Kakui, Frank Uhlmann
Structural maintenance of chromosomes (SMC) complexes play pivotal roles in controlling chromatin organization. Condensin is an essential SMC complex that compacts chromatin to form condensed chromosomes in mitosis. Complete condensin inactivation is necessary to reveal how condensin converts interphase chromatin into mitotic chromosomes. Here, we have developed a condensin depletion system in fission yeast that combines transcriptional repression with auxin-inducible protein degradation. This achieves efficient condensin depletion without need for a temperature shift. Our system is useful when studying how condensin contributes to chromosome architecture and is applicable to the study of other SMC complexes.


Crick (Grant ID: 10198, Grant title: Uhlmann FC001198)