jix529.pdf (1.84 MB)
Effect of HIV on the frequency and number of Mycobacterium tuberculosis-specific CD4+ T cells in blood and the airways during latent M. tuberculosis infection
journal contribution
posted on 2020-07-15, 11:08 authored by Rubina Bunjun, Catherine Riou, Andreia P Soares, Narjis Thawer, Tracey L Müller, Agano Kiravu, Zekarias Ginbot, Tolu Oni, Rene Goliath, Barbara Kalsdorf, Florian von Groote-Bidlingmaier, Willem Hanekom, Gerhard Walzl, Robert J Wilkinson, Wendy A BurgersHuman immunodeficiency virus type 1 (HIV) infection substantially increases the risk of developing tuberculosis. There is extensive depletion of Mycobacterium tuberculosis-specific CD4+ T cells in blood during early HIV infection, but little is known about responses in the lungs at this stage. Given that mucosal organs are a principal target for HIV-mediated CD4+ T-cell destruction, we investigated M. tuberculosis-specific responses in bronchoalveolar lavage (BAL) from persons with latent M. tuberculosis infection and untreated HIV coinfection with preserved CD4+ T-cell counts. M. tuberculosis-specific CD4+ T-cell cytokine (interferon γ, tumor necrosis factor α, and interleukin 2) responses were discordant in frequency and function between BAL and blood. Responses in BAL were 15-fold lower in HIV-infected persons as compared to uninfected persons (P = .048), whereas blood responses were 2-fold lower (P = .006). However, an increase in T cells in the airways in HIV-infected persons resulted in the overall number of M. tuberculosis-specific CD4+ T cells in BAL being similar. Our study highlights the important insights gained from studying M. tuberculosis immunity at the site of disease during HIV infection.
History
Publisher DOI
Usage metrics
Keywords
CD4+ T-cell responsesHuman immunodeficiency virusMycobacterium tuberculosisadaptive immunitybronchoalveolar lavagecoinfectionlungAdultBloodBronchoalveolar Lavage FluidCD4-Positive T-LymphocytesCoinfectionFemaleHIV InfectionsHumansInterferon-gammaInterleukin-2Latent TuberculosisLungMaleTumor Necrosis Factor-alphaYoung AdultWilkinson, R FC00121811 Medical and Health Sciences06 Biological SciencesMicrobiology