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Dendritic cell lineage potential in human early hematopoietic progenitors
journal contributionposted on 2020-08-20, 11:18 authored by Julie Helft, Fernando Anjos-Afonso, Annemarthe G van der Veen, Probir Chakravarty, Dominique Bonnet, Caetano Reis e Sousa
Conventional dendritic cells (cDCs) are thought to descend from a DC precursor downstream of the common myeloid progenitor (CMP). However, a mouse lymphoid-primed multipotent progenitor has been shown to generate cDCs following a DC-specific developmental pathway independent of monocyte and granulocyte poiesis. Similarly, here we show that, in humans, a large fraction of multipotent lymphoid early progenitors (MLPs) gives rise to cDCs, in particular the subset known as cDC1, identified by co-expression of DNGR-1 (CLEC9A) and CD141 (BDCA-3). Single-cell analysis indicates that over one-third of MLPs have the potential to efficiently generate cDCs. cDC1s generated from CMPs or MLPs do not exhibit differences in transcriptome or phenotype. These results demonstrate an early imprinting of the cDC lineage in human hematopoiesis and highlight the plasticity of developmental pathways giving rise to human DCs.
dendritic cellshematopoiesishumanlymphoid cellsmyeloid cellsAnimalsAntigens, DifferentiationCell DifferentiationDendritic CellsHumansLymphoid Progenitor CellsMiceMice, Inbred NODMice, SCIDMice, TransgenicMultipotent Stem CellsReis e Sousa FC001136Bonnet FC001045CBFC-ack0601 Biochemistry and Cell Biology