The Francis Crick Institute
Current_Progress_and_Challenges_in_Large-Scale_3D_Mitochondria_Instance_Segmentation.pdf (4.52 MB)

Current progress and challenges in large-scale 3D mitochondria instance segmentation.

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journal contribution
posted on 2023-12-07, 11:17 authored by Daniel Franco-Barranco, Zudi Lin, Won-Dong Jang, Xueying Wang, Qijia Shen, Wenjie Yin, Yutian Fan, Mingxing Li, Chang Chen, Zhiwei Xiong, Rui Xin, Hao Liu, Huai Chen, Zhili Li, Jie Zhao, Xuejin Chen, Constantin Pape, Ryan Conrad, Luke Nightingale, Joost de Folter, Martin L Jones, Yanling Liu, Dorsa Ziaei, Stephan Huschauer, Ignacio Arganda-Carreras, Hanspeter Pfister, Donglai Wei
In this paper, we present the results of the MitoEM challenge on mitochondria 3D instance segmentation from electron microscopy images, organized in conjunction with the IEEE-ISBI 2021 conference. Our benchmark dataset consists of two large-scale 3D volumes, one from human and one from rat cortex tissue, which are 1,986 times larger than previously used datasets. At the time of paper submission, 257 participants had registered for the challenge, 14 teams had submitted their results, and six teams participated in the challenge workshop. Here, we present eight top-performing approaches from the challenge participants, along with our own baseline strategies. Posterior to the challenge, annotation errors in the ground truth were corrected without altering the final ranking. Additionally, we present a retrospective evaluation of the scoring system which revealed that (1) the challenge metric was permissive with the false positive predictions and (2) the size-based grouping of instances did not correctly categorize mitochondria of interest. Thus, we propose a new scoring system that better reflects the correctness of the segmentation results. Although several of the top methods are compared favorably to our own baselines, substantial errors remain unsolved for mitochondria with challenging morphologies. Thus, the challenge remains open for submission and automatic evaluation, with all volumes available for download.


Crick (Grant ID: CC1076, Grant title: STP Electron Microscopy) Crick (Grant ID: CC1119, Grant title: STP Scientific Computing)