The Francis Crick Institute
ofad128 (1).pdf (1.68 MB)

Blood and site of disease inflammatory profiles differ in patients with pericardial tuberculosis and human immunodeficiency virus type 1

Download (1.68 MB)
journal contribution
posted on 2023-04-06, 10:42 authored by Hygon Mutavhatsindi, Elsa Du Bruyn, Sheena Ruzive, Patrick Howlett, Maddalena Cerrone, Alan Sher, Katrin D Mayer-Barber, Daniel L Barber, Mpiko Ntsekhe, Robert J Wilkinson, Catherine Riou
Objectives To better understand the pathogenesis of pericardial tuberculosis (PCTB), we sought to characterize the systemic inflammatory profile in people with human immunodeficiency virus (HIV-1) with latent TB infection (LTBI), pulmonary TB (PTB) and PCTB. Methods Using Luminex, we measured the concentration of 39 analytes in pericardial fluid (PCF) and paired plasma from 18 PCTB participants, and plasma from 16 LTBI and 20 PTB. Follow-up plasma samples were also obtained from PTB and PCTB participants. HLA-DR expression on Mtb-specific CD4 T cells was measured in baseline samples using flow cytometry. Results Assessment of the overall systemic inflammatory profile by principal component analysis showed that the inflammatory profile of active TB participants was distinct from the LTBI group, while PTB patients could not be distinguished from those with PCTB. When comparing the inflammatory profile between PCF and paired blood, we found that the concentrations of most analytes (24/39) were elevated at site of disease. However, the inflammatory profile in PCF partially mirrored inflammatory events in the blood. After TB treatment completion, the overall plasma inflammatory profile reverted to those observed in the LTBI group. Lastly, HLA-DR expression showed the best performance for TB diagnosis compared to previously described biosignatures built from soluble markers. Conclusion Our results show that the inflammatory profile in blood was comparable between PTB and PCTB. However, at the site of infection (PCF), inflammation was significantly elevated compared to blood. Additionally, our data emphasize the potential role of HLA-DR expression as a biomarker for TB diagnosis.


Crick (Grant ID: 10218, Grant title: Wilkinson, R FC001218)