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An analog sensitive allele permits rapid and reversible chemical inhibition of PKC-3 activity in C. elegans.
journal contributionposted on 2022-08-30, 10:17 authored by KangBo Ng, Tom Bland, Nisha Hirani, Nathan W Goehring
Engineered analog sensitive kinases provide a highly effective method for acute, controllable, and highly selective inhibition of kinase activity. Here we describe the design and characterization of an analog sensitive allele of the polarity kinase, PKC-3. This allele supports normal function as measured by its ability to exclude PAR-2 from the anterior membrane of zygotes, and is rapidly and reversibly inhibited in a dose-dependent manner by the ATP analog 1NA-PP1. This allele provides a new tool to explore the role of PKC-3 in diverse contexts within C. elegans , particularly those in which acute and reversible control of PKC-3 kinase activity may be desired.