The Francis Crick Institute
journal.pgen.1008898.pdf (1.08 MB)

Advances and challenges in genetic technologies to produce single-sex litters.

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journal contribution
posted on 2020-10-22, 10:36 authored by Charlotte Douglas, James MA Turner
There is currently a requirement for single-sex litters for many applications, including agriculture, pest control, and reducing animal culling in line with the 3Rs principles: Reduction, Replacement, and Refinement. The advent of CRISPR/Cas9 genome editing presents a new opportunity with which to potentially generate all-female or all-male litters. We review some of the historical nongenetic strategies employed to generate single-sex litters and investigate how genetic and genome editing techniques are currently being used to produce all-male or all-female progeny. Lastly, we speculate on future technologies for generating single-sex litters and the possible associated challenges.


Crick (Grant ID: 10193, Grant title: Turner FC001193) European Research Council (Grant ID: 647971 - Xchromosome, Grant title: ERC 647971 - Xchromosome)