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A malaria parasite phospholipase facilitates efficient asexual blood stage egress.

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journal contribution
posted on 2023-07-13, 10:21 authored by Abhinay Ramaprasad, Paul-Christian Burda, Konstantinos Koussis, James A Thomas, Emma Pietsch, Enrica Calvani, Steven A Howell, James I MacRae, Ambrosius P Snijders, Tim-Wolf Gilberger, Michael J Blackman
Malaria parasite release (egress) from host red blood cells involves parasite-mediated membrane poration and rupture, thought to involve membrane-lytic effector molecules such as perforin-like proteins and/or phospholipases. With the aim of identifying these effectors, we disrupted the expression of two Plasmodium falciparum perforin-like proteins simultaneously and showed that they have no essential roles during blood stage egress. Proteomic profiling of parasite proteins discharged into the parasitophorous vacuole (PV) just prior to egress detected the presence in the PV of a lecithin:cholesterol acyltransferase (LCAT; PF3D7_0629300). Conditional ablation of LCAT resulted in abnormal egress and a reduced replication rate. Lipidomic profiles of LCAT-null parasites showed drastic changes in several phosphatidylserine and acylphosphatidylglycerol species during egress. We thus show that, in addition to its previously demonstrated role in liver stage merozoite egress, LCAT is required to facilitate efficient egress in asexual blood stage malaria parasites.

Funding

Crick (Grant ID: CC2129, Grant title: Blackman CC2129) Crick (Grant ID: CC1067, Grant title: STP Metabolomics) Crick (Grant ID: CC1063, Grant title: STP Proteomics)

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