jem_20170852.pdf (2.89 MB)
A20-binding inhibitor of NF-κB (ABIN) 2 negatively regulates allergic airway inflammation
journal contributionposted on 2020-09-14, 10:47 authored by Sonia Ventura, Florencia Cano, Yashaswini Kannan, Felix Breyer, Michael J Pattison, Mark S Wilson, Steven C Ley
TPL-2 MAP 3-kinase promotes inflammation in numerous mouse disease models and is an attractive anti-inflammatory drug target. However, TPL-2–deficient (Map3k8−/−) mice develop exacerbated allergic airway inflammation to house dust mite (HDM) compared with wild type controls. Here, we show that Map3k8D270A/D270A mice expressing kinase dead TPL-2 had an unaltered response to HDM, indicating that the severe airway inflammation observed in Map3k8−/− mice is not due to blockade of TPL-2 signaling and rather reflects a TPL-2 adaptor function. Severe allergic inflammation in TPL-2–deficient mice was likely due to reduced levels of ABIN-2 (TNIP2), whose stability depends on TPL-2 expression. Tnip2E256K knock-in mutation, which reduced ABIN-2 binding to A20, augmented the HDM-induced airway inflammation, but did not affect TPL-2 expression or signaling. These results identify ABIN-2 as a novel negative regulator of allergic airway responses and importantly indicate that TPL-2 inhibitors would not have unwanted allergic comorbidities.
Adaptor Proteins, Signal TransducingAnimalsGene Expression Regulation, EnzymologicHypersensitivityInflammationMAP Kinase Kinase KinasesMAP Kinase Signaling SystemMiceMice, KnockoutProto-Oncogene ProteinsTumor Necrosis Factor alpha-Induced Protein 3Ley FC001103FC-ackBRF-ackHP-ackImmunology11 Medical and Health Sciences