SEM image with cells of interest (B cells – blue, T cells – red, Tumour cells – green) identified by confocal fluorescence analysis. Raw images from NanoSIMS showing isotope ratios for the B cell and T cell that are denoted in the SEM image. This data is associated with the manuscript - Unveiling Tissue Microenvironment Dynamics: A Multimodal Imaging Pipeline to Decipher Cell-Specific Metabolic Functions.