Data associated with "Effects of M. tuberculosis and HIV-1 infection on in vitro blood-brain barrier function" paper

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Below is all the information you will need to understand the raw data associated with the paper:

**Effects of M. tuberculosis and HIV-1 infection on in vitro blood-brain barrier function**

*Alizé Proust, Katalin A. Wilkinson, Robert J. Wilkinson*

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**Figure 1 and Additional file 1:**

This folder contains all data used for Figure 1 and Additional file 1.

The file names indicate the purpose of the experiment (HIV infection, Mtb entry of Mtb growth) and the cell type (astrocytes, hCMEC/d3, HBVP or HMC3).

Each file contains all the data organised per experiment (TB-HIV#1 to TB-HIV#7).

**Figure 2:**

This folder contains all data used for Figure 2.

- Figure 2A-B - Dextran-Rhodamine : it includes fluorescence readings of dextran rhodamine B per experiment at 2 and 6 days post-infection (TB-HIV#4 to TB-HIV#12)

- Figure 2C-D - CFU: it includes CFUs count per experiment at 2 and 6 days post-infection (TB-HIV#4 to TB-HIV#12).

**Figure 3:**

This folder contains all data used for Figure 3.

The file names indicate the cell type (astrocytes, hCMEC/d3, HBVP or HMC3).

Each file contains all the data organised per experiment (TB-HIV#1 to TB-HIV#15).

**Figure 4 and Additional file 5:**

This folder contains all data used for Figure 4 and Supplementary Figure 5.

- Subfolder Figure 4A-D : it includes the absorbance readings per cell type (TB-HIV#1 to TB-HIV#7).

- Subfolder Figure 4E-H (and Additional file 5): it includes the luminescence readings per cell type. Each file contains all the data organised per experiment (TB-HIV#1 to TB-HIV#7).

The file names indicate the type of experiment (MTS assay or ROS assay) and the cell type.

**Figure 5:**

This folder contains all data used for Figure 5.

- Figure 5A-B - Astrogliosis: it contains all the qPCR data organised per experiment (TB-HIV#1 to TB-HIV#7).

- Subfolder Figure 5C-H: it includes the fluorescence readings of glutamate organised per cell type (astrocytes, hCMEC/d3, HBVP or HMC3). Each file contains all the data organised per experiment (TB-HIV#1 to TB-HIV#12).

**Figure 6 and Additional file 7:**

This folder contains all Luminex data in pg/mL and stats used for Figure 6 and Additional file 7.

The file names indicate the cell type (astrocytes, hCMEC/d3, HBVP or HMC3).

Each file contains all the data organised per cytokine.

**Additional file 4:**

This folder contains all data used for Additional file 4.

it contains all the qPCR data organised per experiment (TB-HIV#1 to TB-HIV#6).

**Additional file 6:**

This folder contains all data used for Additional file 6. it contains all the qPCR data organised per cell type (astrocytes, hCMEC/d3, HBVP or HMC3).

Each files contains all the qPCR data organised per experiment (TB-HIV#1 to TB-HIV#6).