Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.

Thomas, Adam M; Brolih, Sanja; McGouran, Joanna F; El-Sagheer, Afaf H; Ptchelkine, Denis; Jones, Morgan; McDonald, Neil Q; McHugh, Peter J; Brown, Tom

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Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.

journal contribution

posted on 2020-01-15, 16:51 authored by Adam M Thomas, Sanja Brolih, Joanna F McGouran, Afaf H El-Sagheer, Denis Ptchelkine, Morgan Jones, Neil Q McDonald, Peter J McHugh, Tom Brown

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.