Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

Lanyon-Hogg, Thomas; Ritzefeld, Markus; Sefer, Lea; Bickel, Jasmine K; Rudolf, Amalie F; Panyain, Nattawadee; Bineva-Todd, Ganka; Ocasio, Cory A; O'Reilly, Nicola; Siebold, Christian; Magee, Anthony I; Tate, Edward W

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Acylation-coupled lipophilic induction of polarisation (Acyl-cLIP): a universal assay for lipid transferase and hydrolase enzymes

journal contribution

posted on 2020-01-15, 16:51 authored by Thomas Lanyon-Hogg, Markus Ritzefeld, Lea Sefer, Jasmine K Bickel, Amalie F Rudolf, Nattawadee Panyain, Ganka Bineva-Todd, Cory A Ocasio, Nicola O'Reilly, Christian Siebold, Anthony I Magee, Edward W Tate

A highly accurate and versatile fluorescence polarisation assay for any enzyme adding or removing lipid posttranslational modifications, with the potential to accelerate drug discovery against these targets.