10779/crick.12746225.v1
Akiko Hori
Akiko
Hori
Agathe Morand
Agathe
Morand
Chiho Ikebe
Chiho
Ikebe
David Frith
David
Frith
Ambrosius P Snijders
Ambrosius P
Snijders
Takashi Toda
Takashi
Toda
The conserved Wdr8-hMsd1/SSX2IP complex localises to the centrosome and ensures proper spindle length and orientation
The Francis Crick Institute
2020
Bipolar spindle
Centriolar satellites
Centriole
Centrosome
Fission yeast
Spindle pole body (SPB)
Super resolution microscopy
WD repeats
Cell Line, Tumor
Gene Knockdown Techniques
HeLa Cells
Humans
Microtubule-Associated Proteins
Mitosis
Nuclear Proteins
Proteins
Spindle Apparatus
Hela Cells
Toda
PRT
LM-ack
0304 Medicinal and Biomolecular Chemistry
0601 Biochemistry and Cell Biology
1101 Medical Biochemistry and Metabolomics
Biochemistry & Molecular Biology
2020-07-31 13:25:53
Journal contribution
https://crick.figshare.com/articles/journal_contribution/The_conserved_Wdr8-hMsd1_SSX2IP_complex_localises_to_the_centrosome_and_ensures_proper_spindle_length_and_orientation/12746225
The centrosome plays a pivotal role in a wide range of cellular processes and its dysfunction is causally linked to many human diseases including cancer and developmental and neurological disorders. This organelle contains more than one hundred components, and yet many of them remain uncharacterised. Here we identified a novel centrosome protein Wdr8, based upon the structural conservation of the fission yeast counterpart. We showed that Wdr8 constitutively localises to the centrosome and super resolution microscopy uncovered that this protein is enriched at the proximal end of the mother centriole. Furthermore, we identified hMsd1/SSX2IP, a conserved spindle anchoring protein, as one of Wdr8 interactors by mass spectrometry. Wdr8 formed a complex and partially colocalised with hMsd1/SSX2IP. Intriguingly, knockdown of Wdr8 or hMsd1/SSX2IP displayed very similar mitotic defects, in which spindle microtubules became shortened and misoriented. Indeed, Wdr8 depletion resulted in the reduced recruitment of hMsd1/SSX2IP to the mitotic centrosome, though the converse is not true. Together, we propose that the conserved Wdr8-hMsd1/SSX2IP complex plays a critical role in controlling proper spindle length and orientation.