Gordon, Oliver Henry, Conor M Srinivasan, Naren Ahrens, Susan Franz, Anna Deddouche, Safia Chakravarty, Probir Phillips, David George, Roger Kjaer, Svend Frith, David Snijders, Ambrosius P Valente, Rita S da Silva, Carolina J Simoes Teixeira, Luis Thompson, Barry Dionne, Marc S Wood, Will Reis e Sousa, Caetano α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent genes (Srinivasan et al., 2016). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila. D. melanogaster;DAMP;JAK/STAT pathway;damage-associated molecular pattern;immunology;inflammation;innate immunity;sterile inflammation;tissue injury;Actinin;Actins;Animals;Animals, Genetically Modified;Drosophila Proteins;Drosophila melanogaster;Gene Expression Regulation;Recombinant Proteins;STAT Transcription Factors;Signal Transduction;Reis e Sousa FC001136;Thompson FC001180;CB;GEP;SB;PRT;FLY-ack;0601 Biochemistry and Cell Biology 2020-07-27
    https://crick.figshare.com/articles/journal_contribution/_-actinin_accounts_for_the_bioactivity_of_actin_preparations_in_inducing_STAT_target_genes_in_Drosophila_melanogaster/12696839