10779/crick.12681785.v1 Peter Draber Peter Draber Sebastian Kupka Sebastian Kupka Matthias Reichert Matthias Reichert Helena Draberova Helena Draberova Elodie Lafont Elodie Lafont Diego de Miguel Diego de Miguel Lisanne Spilgies Lisanne Spilgies Silvia Surinova Silvia Surinova Lucia Taraborrelli Lucia Taraborrelli Torsten Hartwig Torsten Hartwig Eva Rieser Eva Rieser Luigi Martino Luigi Martino Katrin Rittinger Katrin Rittinger Henning Walczak Henning Walczak LUBAC-recruited CYLD and A20 regulate gene activation and cell death by exerting opposing effects on linear ubiquitin in signaling complexes The Francis Crick Institute 2020 Cell Death Cell Line DNA-Binding Proteins Deubiquitinating Enzyme CYLD Humans Immunoprecipitation Intracellular Signaling Peptides and Proteins Nuclear Proteins Signal Transduction Transcriptional Activation Transduction, Genetic Tumor Necrosis Factor alpha-Induced Protein 3 Tumor Suppressor Proteins Ubiquitin Ubiquitin-Protein Ligase Complexes Ubiquitination Rittinger U117565398 0601 Biochemistry and Cell Biology 2020-07-21 10:11:13 Journal contribution https://crick.figshare.com/articles/journal_contribution/LUBAC-recruited_CYLD_and_A20_regulate_gene_activation_and_cell_death_by_exerting_opposing_effects_on_linear_ubiquitin_in_signaling_complexes/12681785 Ubiquitination and deubiquitination are crucial for assembly and disassembly of signaling complexes. LUBAC-generated linear (M1) ubiquitin is important for signaling via various immune receptors. We show here that the deubiquitinases CYLD and A20, but not OTULIN, are recruited to the TNFR1- and NOD2-associated signaling complexes (TNF-RSC and NOD2-SC), at which they cooperate to limit gene activation. Whereas CYLD recruitment depends on its interaction with LUBAC, but not on LUBAC's M1-chain-forming capacity, A20 recruitment requires this activity. Intriguingly, CYLD and A20 exert opposing effects on M1 chain stability in the TNF-RSC and NOD2-SC. While CYLD cleaves M1 chains, and thereby sensitizes cells to TNF-induced death, A20 binding to them prevents their removal and, consequently, inhibits cell death. Thus, CYLD and A20 cooperatively restrict gene activation and regulate cell death via their respective activities on M1 chains. Hence, the interplay between LUBAC, M1-ubiquitin, CYLD, and A20 is central for physiological signaling through innate immune receptors.