Real-time dynamics of Plasmodium NDC80 reveals unusual modes of chromosome segregation during parasite proliferation. Mohammad Zeeshan Rajan Pandey David JP Ferguson Eelco C Tromer Robert Markus Steven Abel Declan Brady Emilie Daniel Rebecca Limenitakis Andrew R Bottrill Karine G Le Roch Anthony A Holder Ross F Waller David S Guttery Rita Tewari 10779/crick.12661499.v1 https://crick.figshare.com/articles/journal_contribution/Real-time_dynamics_of_Plasmodium_NDC80_reveals_unusual_modes_of_chromosome_segregation_during_parasite_proliferation_/12661499 Eukaryotic cell proliferation requires chromosome replication and precise segregation to ensure daughter cells have identical genomic copies. The genus Plasmodium, the causative agent of malaria, displays remarkable aspects of nuclear division throughout its lifecycle to meet some peculiar and unique challenges of DNA replication and chromosome segregation. The parasite undergoes atypical endomitosis and endoreduplication with an intact nuclear membrane and intranuclear mitotic spindle. To understand these diverse modes of Plasmodium cell division, we have studied the behaviour and composition of the outer kinetochore NDC80 complex, a key part of the mitotic apparatus that attaches the centromere of chromosomes to microtubules of the mitotic spindle. Using NDC80-GFP live-cell imaging in Plasmodium berghei we observe dynamic spatiotemporal changes during proliferation, including highly unusual kinetochore arrangements during sexual stages. We identify a very divergent candidate for the SPC24 subunit of the NDC80 complex, previously thought to be missing in Plasmodium, which completes a canonical, albeit unusual, NDC80 complex structure. Altogether, our studies reveal the kinetochore as an ideal tool to investigate the non-canonical modes of chromosome segregation and cell division in Plasmodium. 2020-07-17 16:32:36 Atypical cell division Chromosome segregation Endomitosis Kinetochore Malaria Meiosis NDC80 complex Plasmodium Holder FC001097 Developmental Biology 06 Biological Sciences 11 Medical and Health Sciences