Human axial progenitors generate trunk neural crest cells in vitro
Thomas JR Frith
Ilaria Granata
Matthew Wind
Erin Stout
Oliver Thompson
Katrin Neumann
Dylan Stavish
Paul R Heath
Daniel Ortmann
James OS Hackland
Konstantinos Anastassiadis
Mina Gouti
James Briscoe
Valerie Wilson
Stuart L Johnson
Marysia Placzek
Mario R Guarracino
Peter W Andrews
Anestis Tsakiridis
10779/crick.12652742.v1
https://crick.figshare.com/articles/journal_contribution/Human_axial_progenitors_generate_trunk_neural_crest_cells_in_vitro/12652742
The neural crest (NC) is a multipotent embryonic cell population that generates distinct cell types in an axial position-dependent manner. The production of NC cells from human pluripotent stem cells (hPSCs) is a valuable approach to study human NC biology. However, the origin of human trunk NC remains undefined and current in vitro differentiation strategies induce only a modest yield of trunk NC cells. Here we show that hPSC-derived axial progenitors, the posteriorly-located drivers of embryonic axis elongation, give rise to trunk NC cells and their derivatives. Moreover, we define the molecular signatures associated with the emergence of human NC cells of distinct axial identities in vitro. Collectively, our findings indicate that there are two routes toward a human post-cranial NC state: the birth of cardiac and vagal NC is facilitated by retinoic acid-induced posteriorisation of an anterior precursor whereas trunk NC arises within a pool of posterior axial progenitors.
2020-07-15 11:18:22
In vitro differentiation
axial progenitors
developmental biology
embryonic development
human
neural crest
neuromesodermal progenitors
pluripotent stem cells
regenerative medicine
stem cells
Biomarkers
Cell Differentiation
Cells, Cultured
Humans
Neural Crest
Pluripotent Stem Cells
Briscoe FC001051
0601 Biochemistry and Cell Biology